Recovery and functional validation of hidden soil enzymes in metagenomic libraries
نویسندگان
چکیده
منابع مشابه
Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms.
Landfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collected from Ilhéus, BA, Brazil, was extract...
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Recent advances in sequencing technologies generate new predictions and hypotheses about the functional roles of environmental microorganisms. Yet, until we can test these predictions at a scale that matches our ability to generate them, most of them will remain as hypotheses. Function-based mining of metagenomic libraries can provide direct linkages between genes, metabolic traits and microbia...
متن کاملNew carbohydrate-active enzymes identified by screening two metagenomic libraries derived from the soil of a winter wheat field.
AIMS Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing two metagenomic DNA libraries from soil samples collected in winter and spring from a field of winter wheat. METHODS AND RESULTS Both libraries were screened on esculin-supplemented medium s...
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in fact, this study focused on the following questions: 1. is there any difference between the effect of functional/notional approach and the structural approaches to language teaching on the proficiency test of efl learners? 2. can a rather innovative language test referred to as "functional test" ge devised so so to measure the proficiency test of efl learners, and thus be as much reliable an...
15 صفحه اولLarge-scale screens of metagenomic libraries.
Metagenomic libraries archive large fragments of contiguous genomic sequences from microorganisms without requiring prior cultivation. Generating a streamlined procedure for creating and screening metagenomic libraries is therefore useful for efficient high-throughput investigations into the genetic and metabolic properties of uncultured microbial assemblages. Here, key protocols are presented ...
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ژورنال
عنوان ژورنال: MicrobiologyOpen
سال: 2019
ISSN: 2045-8827,2045-8827
DOI: 10.1002/mbo3.572